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| TITLE | Analysis of Fatty Acids in Rice Bran Oil, Coconut Oil and Margarine by HPLC-ELSD |
|---|---|
| AUTHOR | JASCO Corp. |
| YEAR | |
| JOURNAL | JASCO Corp. |
| ABSTRACT | Due to its poor UV absorbance, fatty acids have been commonly measured at a short UV wavelengths or using a refractive index detector – which can take a long time to establish a stable baseline. Pre-column derivatization is another well known approach in the analysis of fatty acids, but the procedure is complicated. ELSD is recognized as an effective method for lipid analysis providing high sensitivity and a stable baseline while also eliminating complex sample pre-preparation. As the sample becomes more volatile, with a shorter carbon chain, ELSD is normally considered inadequate and difficult to measure the fatty acid. This report describes analysis using an ELSD capable of low temperature measurement, for saturated and unsaturated fatty acids with chain lengths C10 to C18. |
| FULL ARTICLE | https://jascoinc.com/wp-content/uploads/2014/01/Analysis-of-fatty-acids-in-rice-bran-oil-coconut-oil-and-margarine-by-HPLC-ELSD.pdf |
| INSTRUMENT | HPLC, ELSD |
| KEYWORDS | HPLC, ELSD, Oil |
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