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Found 6,756 Results / Page 75 of 451
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Interaction among protein, daidzein and surfactants in the WPI-based daidzein self-microemulsifying delivery system
2020 / Food ChemistryThe self-microemulsifying delivery system was fabricated by whey protein isolate (WPI), daidzein (Dai) and surfactants, the interaction of WPI, Dai and D-α-Tocopherol polyethylene glycol succinate (TPGS) was hereby studied in the absence or presence of Tween 20. The increase of surfactant concentration led to the decrease of the modulus and changes of protein...
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The isolated armadillo-repeat domain of Plakophilin 1 is a monomer in solution with a low conformational stability
2020 / Journal of Structural BiologyPlakophilin 1 (PKP1) is a member of the armadillo repeat family of proteins. It serves as a scaffold component of desmosomes, which are key structural components for cell–cell adhesion. We have embarked on the biophysical and conformational characterization of the ARM domain of PKP1 (ARM-PKP1) in solution by using several spectroscopic (namely, fluorescence a...
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Functional and structural evaluation of the antileukaemic enzyme L-asparaginase II expressed at low temperature by different Escherichia coli strains
2020 / Biotechnology LettersAcute lymphoblastic leukaemia (ALL) affects lymphoblastic cells and is the most common neoplasm during childhood. Among the pharmaceuticals used in the treatment protocols for ALL, Asparaginase (ASNase) from Escherichia coli (EcAII) is an essential biodrug. Meanwhile, the use of EcAII in neoplastic treatments causes several side effects, such as immunological react...
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The Multifaceted Effects of DMSO and High Hydrostatic Pressure on the Kinetic Constants of Hydrolysis Reactions Catalyzed by α-Chymotrypsin
2020 / Physical Chemistry Chemical PhysicsThe use of cosolvents and high hydrostatic pressure (HHP) has been described as an efficient means to modulate the stability of enzymes and their catalytic activity. Cosolvents and pressure can lead to increased reaction rates without affecting the stability of the enzyme. Here, we studied the combined effects of one of the most used organic cosolvents, dimethyl su...
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Thioflavin T as a noncovalent reporter for a label-free, non-enzymatic, catalytic DNA amplifier
2020 / Methods and Applications in FluorescenceDNA-DNA reactions can be monitored with a label-free fluorogenic reaction. Guanosine-rich, single-stranded DNA oligonucleotides bind to thioflavin-T (ThT) and enhance the fluorescence of the dye. We discovered a novel DNA sequence that produces fluorescence upon binding to ThT. We denote this oligonucleotide ThTSignal. We use ThTSignal as a label-free reporter for ...
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Probe into a multi-protein prokaryotic organelle using thermal scanning assay reveals distinct properties of the core and the shell
2020 / Biochimica et Biophysica Acta (BBA) - General SubjectsBacterial microcompartment represents the only reported category of prokaryotic organelles that are capable of functioning as independent bioreactors. In this organelle, a biochemical pathway with all the enzyme machinery is encapsulated within an all protein shell. The shell proteins and the enzymes have distinct structural features. It is hypothesized that flat s...
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Amylin and beta amyloid proteins interact to form amorphous heterocomplexes with enhanced toxicity in neuronal cells
2020 / Scientific ReportsHuman pancreatic islet amyloid polypeptide (hIAPP) and beta amyloid (Aβ) can accumulate in Type 2 diabetes (T2D) and Alzheimer’s disease (AD) brains and evidence suggests that interaction between the two amyloidogenic proteins can lead to the formation of heterocomplex aggregates. However, the structure and consequences of the formation of these complexe...
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Functional analysis of BPSS2242 reveals its detoxification role in Burkholderia pseudomallei under salt stress
2020 / Scientific ReportsA bpss2242 gene, encoding a putative short-chain dehydrogenase/oxidoreductase (SDR) in Burkholderia pseudomallei, was identified and its expression was up-regulated by ten-fold when B. pseudomallei was cultured under high salt concentration. Previous study suggested that BPSS2242 plays important roles in adaptation to salt stress and pathogenesis; however, its biol...
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Biochemical Characterization of Lactose Binding Entadin Lectin from Entada rheedii Seeds with Cytotoxic Activity against Cancer Cell Lines
2020 / ACS OmegaA novel Entadin lectin was isolated, purified, and characterized from the seeds of Entada rheedii by ammonium sulfate precipitation, followed by lactose affinity chromatography. On sodium dodecyl sulfate polyacrylamide gel electrophoresis, the purified Entadin lectin appeared as a single band (monomeric in nature) with a molecular mass of approximately 20 kDa in bo...
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Peroxynitrous acid (ONOOH) modifies the structure of Anastellin and influences its capacity to polymerize fibronectin
2020 / Redox BiologyAnastellin (AN), a fragment of the first type III module in fibronectin (FN), initiates formation of superfibronectin, a polymer which resembles the native cell-derived fibrillar FN found in the extracellular matrix of many tissues, but which displays remarkably different functional properties. Here we demonstrate that exposure of AN to the biologically-important i...
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Thermodynamic analysis of albumin interaction with monosodium glutamate food additive: Insights from multi-spectroscopic and molecular docking approaches
2020 / Journal of Molecular StructureMonosodium glutamate (MSG) is an example of food additive, which is used as a flavor enhancer in various foodstuffs. Because of its extensive use in food production, the toxicology and possible negative effects of MSG additive on albumin must be well investigated. Therefore, for the first time, the binding of BSA and MSG have been studied using multispectroscopic a...
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Enzymatic versatility and thermostability of a new aryl-alcohol oxidase from Thermothelomyces thermophilus M77
2020 / Biochimica et Biophysica Acta (BBA) - General SubjectsFungal aryl-alcohol oxidases (AAOx) are extracellular flavoenzymes that belong to glucose-methanol-choline oxidoreductase family and are responsible for the selective conversion of primary aromatic alcohols into aldehydes and aromatic aldehydes to their corresponding acids, with concomitant production of hydrogen peroxide (H2O2) as by-product. The H2O2 can be provi...
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Plasmodium vivax AMA1: Implications of distinct haplotypes for immune response
2020 / PLOS Neglected Tropical DiseasesIn Brazil, Plasmodium vivax infection accounts for around 80% of malaria cases. This infection has a substantial impact on the productivity of the local population as the course of the disease is usually prolonged and the development of acquired immunity in endemic areas takes several years. The recent emergence of drug-resistant strains has intensified research on...
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Thermogelling Inclusion Complex System for Fine-Tuned Osteochondral Differentiation of Mesenchymal Stem Cells
2020 / BiomacromoleculesHow to control osteochondral differentiation of mesenchymal stem cells at a proper stage is a key issue for articular cartilage regeneration. To solve this problem, injectable scaffolds with different chemical functional groups were designed by introducing one equivalent of α-cyclodextrin (α-CD) carboxylate and α-CD phosphate along poly(ethylene g...
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Structure-activity analysis of truncated albumin-binding domains suggests new lead constructs for potential therapeutic delivery
2020 / Journal of Biological ChemistryRapid clearance by renal filtration is a major impediment to the translation of small bioactive biologics into drugs. To extend serum half-life, a commonly used approach is to attach drug leads to the G-related albumin-binding domain (ABD) to bind albumin and evade clearance. Despite the success of this approach in extending half-lives of a wide range of biologics,...
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