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Found 6,446 Results / Page 430 of 430
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Termitomycesphins A–D, Novel Neuritogenic Cerebrosides from the Edible Chinese Mushroom Termitomyces albuminosus
2000 / TetrahedronFour novel cerebrosides, termitomycesphins A–D, were isolated from the edible Chinese mushroom Termitomyces albuminosus (Berk.) Heim. (‘Jizong’ in Chinese), shown to induce neuronal differentiation in rat PC12 cells. The absolute stereostructures were elucidated by spectroscopic methods and chemical derivatization. These new cerebrosides have a un...
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BSA structural changes during homomolecular exchange between the adsorbed and the dissolved states
2000 / Journal of BiotechnologyThe secondary structure and the thermostability of bovine serum albumin (BSA), before adsorption and after homomolecular displacement from silica and polystyrene particles, are studied by circular dichroism spectroscopy and differential scanning calorimetry. The structural perturbations induced by the hydrophilic silica surface are reversible, i.e. BSA completely r...
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Selected Mutations in a Mesophilic Cytochrome cConfer the Stability of a Thermophilic Counterpart
2000 / The Journal of Biological ChemistryMesophilic cytochrome c551 of Pseudomonas aeruginosa (PA c551) became as stable as its thermophilic counterpart, Hydrogenobacter thermophilus cytochrome c552 (HT c552), through only five amino acid substitutions. The five residues, distributed in three spatially separated regions, were selected and mutated with reference to the corresponding residues in HT c552 thr...
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Fiber diffraction of synthetic a-synuclein filaments shows amyloid-like cross-b conformation
2000 / PNASFilamentous inclusions made of a-synuclein constitute the defining neuropathological characteristic of Parkinson’s disease, dementia with Lewy bodies, and multiple system atrophy. Rare familial cases of Parkinson’s disease are associated with mutations A53T and A30P in a-synuclein. We report here the assembly properties and secondary structure character...
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Conformation of the RNA Polymerase II C-Terminal Domain: Circular Dichroism of Long and Short Fragments
2000 / The Journal of Molecular BiologyThe C-terminal domain (CTD) of the largest subunit of RNA polymerase II consists of tandemly repeated copies of a heptapeptide with the Y1 S2 P3 T4 S5 P6 S7 consensus sequence. This repeat contains two overlapping SPXX motifs that can adopt a b-turn conformation. In addition, each CTD repeat contains the PXXP sequence characteristic of the left-handed helix of poly...
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Protein stabilising effect of polyethyleneimine
1999 / Journal of BiotechnologyThe effect of polyethyleneimine (PEI), a cationic polymer, on protein stability was investigated. Shelf stability of lactate dehydrogenase, stored as an aqueous solution, at 36°C was distinctly improved in the presence of 0.01–1% (w/v) polymer (except at pH 5.0). PEI also prevented aggregation of the protein during long-term storage. Both high and low mol...
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Synthesis and Structural Characterization of Helix-Forming â-Peptides: trans-2-Aminocyclopentanecarboxylic Acid Oligomers
1999 / JACSSynthetic protocols and circular dichroism (CD) spectra are reported for a series of oligomers of (R,R)-trans-2-aminocyclopentanecarboxylic acid (trans-ACPC). The two longest oligomers, a hexamer and an octamer, have also been examined crystallographically. Both crystal structures show that the â-peptide backbone adopts a regular helix that is defined by a se...
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Submillisecond protein folding kinetics studied by ultrarapid mixing
1997 / PNASAn ultrarapid-mixing continuous-flow method has been developed to study submillisecond folding of chemically denatured proteins. Turbulent flow created by pumping solutions through a small gap dilutes the denaturant in tens of microseconds. We have used this method to study cytochrome c folding kinetics in the previously inaccessible time range 80 μs to 3 ms. To...
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Thermoresponsive Dendronized Polypeptides Showing Switchable Recognition to Catechols
2016 / MacromoleculesA new class of thermoresponsive dendronized polypeptides was prepared through highly efficient oxime ligation between oxyamino-substituted polylysines and aldehyde-cored oligoethylene glycol (OEG) dendrons. Their secondary structures and thermoresponsive behavior were investigated. Because of the dendritic structures and stable oxime linkage, these OEG-based dendro...
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The Pathogenic Mutation T182A Converts the Prion Protein into a Molten Globule-like Conformation Whose Misfolding to Oligomers but Not to Fibrils Is Drastically Accelerated
2016 / BiochemistryDelineation of the effects of pathogenic mutations linked with familial prion diseases on the structure and misfolding of prion protein (PrP) will be useful in understanding the molecular mechanism of PrP misfolding. Here, it has been shown that the pathogenic mutation T182A causes a drastic reduction in the apparent cooperativity and enthalpy of unfolding of the m...
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Implications of Human Transient Receptor Potential Melastatin 8 (TRPM8) Channel Gating from Menthol Binding Studies of the Sensing Domain
2016 / BiochemistryThe transient receptor potential melastatin 8 (TRPM8) ion channel is the primary cold sensor in humans. TRPM8 is gated by physiologically relevant cold temperatures and chemical ligands that induce cold sensations, such as the analgesic compound menthol. Characterization of TRPM8 ligand-gated channel activation will lead to a better understanding of the fundamental...
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