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Color is a visually perceived property that is derived from the reflected or transmitted spectrum of light interacting with the human eye. It is important for the development and production of color-based products to evaluate and obtain measurable factors that describe the color. JASCO has developed a variety of color analysis using a UV-Visible spectrophotometer ...
Continue readingFluorescence resonance energy transfer (FRET) is a mechanism governing the energy transfer between two neighboring molecules. A donor, initially in its excited state, may transfer energy to an acceptor molecule through non-radiative electron resonance. FRET is monitored using a spectrofluorometer, which measures the fluorescence/quenching of an acceptor or excit...
Continue readingThis application note compares the accuracy of thermal melting measurements of a DNA sample using the temperature sensor inside a cell compared to the block sensor using the the V-630 with PAC-743 . The 8-position micro cell sensors as a temperature monitor (Figure 1), the horizontal axis of the temperature course data can be plotted with actual temperatures obtain...
Continue readingA dichroic mirror is an optical element consisting of multi-layered dielectric materials that have different refractive indexes. This enables the mirror to reflect the light of a specific wavelength and transmit the light of another. Dichroic mirrors have different spectral characteristics, depending on the angle or polarization of the incident light. Therefore, in...
Continue readingObjects that emit light are known as luminous materials and are increasingly being used in products such as Light Emitting Diodes (LED), Organic Electrical Luminescent (EL) Displays, and Plasma Display Panels (PDP). After the introduction of the blue LED in 1993, engineers developed a white LED that is now the mainstream illuminator of the mobile phone backlight an...
Continue readingHeat shield glass is typically made from eco-friendly materials that help in the prevention of global warming and reduction in energy costs. Testing methods for the evaluation of heat shield glass is stipulated by JIS R3016 and require visible transmittance, visible reflectance, solar transmittance, solar reflectance, and emittance measurements. From these measurem...
Continue readingIn recent years, solar reflective paints have attracted attention as a way to combat the ‘heat island’ phenomenon and as a sustainable household building material. Heat islands are urban areas where the temperature is significantly hotter than the surrounding rural areas and occur where buildings, roads, and infrastructure have replaced a majority of th...
Continue readingThe PIV-756 and PIN-757 Horizontal Integrating spheres (Figure 1) are used for measuring the diffuse reflectance and transmittance of contact lenses and other samples. By positioning the samples horizontally, uneven or irregularly shapes can be easily measured. The horizontal integrating sphere allows precise measurement and increases the operating efficiency even ...
Continue readingSpectroscopic Analysis of Sunscreen SPF/PA is made with reference to two rating systems used to assess the protection offered by sunscreens and cosmetics: SPF (Sun Protection Factor) and PA (Protection Grade for UV-A). Both are used to indicate the level of protection provided by skin care products against skin damage from UV radiation. The SPF value represents ...
Continue readingIn this study, we developed a new method for the chiral separation of pesticides using SFC-CD-MS, with a circular dichroism detector (CD) and mass spectrometer (MS). Around 30% of pesticides currently used worldwide are chiral isomers with one or more chiral centers. It is well known that the enantiomers of chiral pesticides have different biological activities, to...
Continue readingA fluorescent powder may be evaluated by its fluorescence spectrum, excitation spectrum, external/internal quantum efficiency and luminescent color. In addition, a diffuse reflectance spectrum may be required for evaluating its absorption spectrum. However, when making diffuse reflectance measurement of fluorescent materials using a UV-visible spectrophotometer w...
Continue readingA fluorometer with optical fiber can be used for a variety of measurements, such as when the sample is larger than the sample chamber, to follow an in-situ reaction, or for measurement in hazardous environments, such as high/low temperature and high pressure. The OBF-832 optical fiber interface is used to connect an optical fiber for remote sampling of fluorescence...
Continue readingTwo dimensional correlation spectroscopy was first proposed in the 1980s as an analytical method for investigating the changes in IR spectra with time. Although 2D correlation spectroscopy is typically used to analyze a single spectral type obtained from a time course measurement, in this experiment, two dimensional correlation spectroscopy was applied to time-cour...
Continue readingNear-infrared fluorescence labels are currently attracting the attention of the scientific community due to their wide range of biological applications. These labels are ideal for biological studies since biomolecules absorb very little in the near-infrared region. NIR fluorescence labels have shown no adverse effects in vivo and are highly stable. This applicat...
Continue readingFor many biological samples, it is much more convenient, cost effective, and efficient to use microvolumes for structural and quantitative studies. The SAF-850 One Drop is a microsampling accessory that enables fluorescence measurements to be obtained with only 5 µL of sample. The highly reproducible baseline allows for either simple spectrum measurements or...
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