JASCO | Stopped-flow measurment
What is stopped-flow measurements
TS Science
Product
[CD] Circular Dichroism
[CD]Stopped-Flow
→ Stopped-flow measurements involve the rapid mixing of two or more solutions to trigger a chemical reaction, the kinetics of which can be followed by CD, absorbance and fluorescence.
All probe methods can be measured on the same instrument when the stopped-flow system is paired with a J-1500 CD spectrometer.
1. SFS-562 | Stopped-Flow System
The SFS-600 series is an innovative stopped-flow measurement accessory with a modular design that allows the flow cell unit to be easily installed and removed from the sample compartment without alignment.
Two-,three-and four-syringe models are available, offering flexible mixing as well as upgradeability for quench-flow. For temperature-dependent kinetic experiments, the options are Peltier temperature-controlled syringes.
Stepper-motor-driven syringes allow infinitely variable mixing ratios and a mechanical mixer efficiently mixes solutions of different viscosities commonly used in protein folding experiments.
• Applications to studies such as protein folding, substrate binding and enzyme kinetics
• Standard 2 mm cell (optional 0.5, 1 and 10 mm cells)
• Standard 10 mL syringe (optional 1, 2.5, and 5 mL syringes)
• 5 mL/sec flow rate with 10 mL syringe
• Exact control of flow rate
• Mixing ratio from 1:1 to 1:20
• Dead time: 0.57 msec with a 2 mm cell
• Peltier temperature control (SFS-602T/SFS-603T/SFS-604T) ranges from 5 to 80°C (with cell) and 5 to 60°C (with syringe)
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