HPLC System

Concept of the Integrated LC-4000 Series HPLC

The LC-4000 Series HPLC has key separation platforms at 50MPa for conventional HPLC (3, 5 and 10 μm columns), at 70MPa for RHPLC (intermediate columns such as 2 to 3 μm and ‘Coreshell’) and at 130MP for UHPLC (sub 2μm).

Advances in Solvent Delivery

For more than three decades JASCO analytical HPLC pumps have employed an asymmetric twin-piston solvent delivery system SSQD (Slow Suction, Quick Delivery) which provides significantly better flow and pressure characteristics than conventional twin-piston reciprocating designs. SSQD Technology offers the highest stability ‘compensated’ solvent delivery used in the PU-4180/85 RHPLC and PU-4280/85 UHPLC pump models, this makes it an excellent choice for detectors that are affected by flow pulsation like ECD, RI and mass spec.

Pioneering Optical Design

As a pioneer in optical spectroscopy, JASCO has optical designs that few can match. We have developed a range of HPLC detectors with unrivaled performance, including the class leading FP-4020 fluorescence detector with  S/N  greater than 2300:1 (Water Raman), RI-4035 UHPLC refractive index detector and the unique CD-4095 circular dichroism detector for chiral chromatography.

HPLC Method Development

With the range of separation columns becoming increasingly diverse – the traditional 5μm fully porous particle is finally losing ground to some of the newer technologies such as the new superficially porous solid core (coreshell) and the wide variety of sub-2μm particles. Requirements for method development are becoming more demanding and time consuming. For method development the CO-4065 prep columns oven can accommodate up to10 analytical, preparative columns with automatic switching, and 6 or 10 solvent selection valves, controlled by the ChromNAV Method Development Module.

Compact and Easy to Use

The footprint  of the LC-4000 Series HPLC is only 30cm – requiring only a very small amount of bench space. For easy maintenance, all LC-4000 instruments feature front-panel access for replacing consumables such as pump seals and check valves and detector light sources. The autosampler consumables such as the syringe parts are easy to access.

Single Module Operation Front Panel Control

For users requiring front-panel control, the LC-4000 Series retains a keypad and backlit LED display. Keypad control can still be configured for convenient override when under PC control.

External Software control

HPLC modules are often used for non-HPLC applications, such as high accuracy feed pumps or stand-alone detectors for monitoring flow and reactors. For unattended operation RS-232 remains a convenient method for control and monitoring, using a simple communication protocol that can be included with each module.